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B6.129P2-Apoetm1Unc/J
品系货号:002052 | 通用名称:ApoE KO 小鼠
此品系 Apoetm1Unc 基因敲除突变纯合小鼠的血浆总胆固醇水平明显升高,且不受鼠龄或性别影响。 3 月龄小鼠的近端主动脉可见脂纹。该病变随鼠龄增大而加剧,逐渐发展为脂质含量更低但细胞更长的病变,这是动脉粥样硬化病变前的后期典型特征。这些 ApoE KO 小鼠适用于研究心血管疾病、动脉粥样硬化和脂肪代谢,以及研究 ApoE 在阿尔茨海默症中的作用。
品系特点
  
靶向突变
  
品系捐赠者 Nobuyo Maeda博士 北卡罗莱纳大学教堂山分校
  
原始参考文献
Info 当使用该小鼠品系发表文献时,请引用原始参考文献,并在材料方法中提供该品系的品系货号:JAX stock #002052
查阅参考文献 >
  
品系详情
此品系Apoetm1Unc 突变纯合小鼠的血浆总胆固醇水平明显升高,且不受鼠龄或性别的影响。 3 月龄小鼠的近端主动脉可见脂纹。该病变随鼠龄增大而加剧,逐渐发展为脂质含量更低但细胞更长的病变,这是动脉粥样硬化病变前的后期典型特征。在 C57BL/6 x 129 混合遗传背景上具有该突变的小鼠甘油三酯水平中度升高。研究显示,apoE 缺陷老龄鼠(> 17 月龄)的大脑中会出现黄色瘤样病变,该病变主要包括结晶胆固醇裂隙、脂质小球和泡沫细胞。脉络丛和腹穹窿中可见较小黄色瘤。另有研究表明,apoE 缺陷小鼠的应激反应发生改变、空间学习和记忆受损、长时程增强效应改变,且突触受损。
品系建立
Apoetm1Unc 突变品系由北卡罗来纳大学教堂山分校的 Nobuyo Maeda 博士实验室建立。在品系建立过程中,使用了源自 129P2/OlaHsd 的 E14Tg2a ES 细胞系。所用质粒命名为 pNMC109,始祖细胞系为主要参考文献中提及的 T-89。此 C57BL/6J 同源 Apoetm1Unc 品系是通过与 C57BL/6J 近交小鼠回交至少 10 代获得的。这些小鼠的被毛呈黑色。
需要注意的是,在 1995 年以前,仅销售与 C57BL/6J 小鼠回交 6 代 (N6) 的小鼠。N6 代小鼠为粉红色眼睛稀释基因 p 的纯合小鼠,该基因使此品系小鼠具有粉红色眼睛和银色被毛。E14Tg2a ES 细胞系携带此种隐性突变,在回交 N6 代时,该突变仍与 7 号染色体上的靶向 Apoe 基因连锁。目前,已不再销售 N6 种群小鼠 [1995]。
参考文献
精选参考文献
当使用该小鼠品系发表文献时,请引用原始参考文献,并在材料方法中提供该品系的品系货号:JAX stock #002052
1992

Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells.

Piedrahita JA , et al.

PubMed:1584779

MGI:J:1050

Proc Natl Acad Sci U S A 89(10):4471-5

基因分型和繁育
Allele Symbol Apoe<tm1Unc>
Allele Specific Protocols
  
此品系繁育表现良好。
繁育策略
纯合子x纯合子
销售和使用条款

查阅销售和使用条款Info > 

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002052
ApoE KO
神经生物学,糖尿病与肥胖症,心血管疾病
参考文献|
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    精选参考文献
    当使用该小鼠品系发表文献时,请引用原始参考文献,并在材料方法中提供该品系的品系货号:JAX stock #002052

    1992
    Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells.
    Piedrahita JA , et al.
    PubMed:1584779
    MGI:J:1050
    Proc Natl Acad Sci U S A 89(10):4471-5

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    其它参考文献

    2004
    Neuron-specific apolipoprotein e4 proteolysis is associated with increased tau phosphorylation in brains of transgenic mice.
    Brecht WJ , et al.
    PubMed:15014128
    MGI:J:90126
    J Neurosci 24(10):2527-34

    2004
    A nontransgenic mouse model shows inducible amyloid-beta (Abeta) peptide deposition and elucidates the role of apolipoprotein E in the amyloid cascade.
    Dolev I , et al.
    PubMed:15365176
    MGI:J:92619
    Proc Natl Acad Sci U S A 101(38):13909-14

    2004
    Effect of macrophage-derived apolipoprotein E on hyperlipidemia and atherosclerosis of LDLR-deficient mice.
    Shi W , et al.
    PubMed:15047172
    MGI:J:88891
    Biochem Biophys Res Commun 317(1):223-9

    2003
    A decreased expression of angiopoietin-like 3 is protective against atherosclerosis in apoE-deficient mice.
    Ando Y , et al.
    PubMed:12671033
    MGI:J:84022
    J Lipid Res 44(6):1216-23

    2003
    Loss of TGF-beta1 leads to increased neuronal cell death and microgliosis in mouse brain.
    Brionne TC , et al.
    PubMed:14687548
    MGI:J:87148
    Neuron 40(6):1133-45

    2003
    Angiotensin II-accelerated atherosclerosis and aneurysm formation is attenuated in osteopontin-deficient mice.
    Bruemmer D , et al.
    PubMed:14597759
    MGI:J:86531
    J Clin Invest 112(9):1318-31

    2003
    Two Hsp70 family members expressed in atherosclerotic lesions.
    Han Z , et al.
    PubMed:12552099
    MGI:J:81835
    Proc Natl Acad Sci U S A 100(3):1256-61

    2003
    Circulating activated platelets exacerbate atherosclerosis in mice deficient in apolipoprotein E.
    Huo Y , et al.
    PubMed:12483207
    MGI:J:81164
    Nat Med 9(1):61-7

    2003
    Impaired electroretinogram (ERG) response in apolipoprotein E-deficient mice.
    Ong JM , et al.
    PubMed:12868005
    MGI:J:84688
    Curr Eye Res 27(1):15-24

    2003
    Central role of RAGE-dependent neointimal expansion in arterial restenosis.
    Sakaguchi T , et al.
    PubMed:12671045
    MGI:J:82620
    J Clin Invest 111(7):959-72

    2003
    Hyperhomocysteinemia accelerates atherosclerosis in cystathionine beta-synthase and apolipoprotein E double knock-out mice with and without dietary perturbation.
    Wang H , et al.
    PubMed:12506016
    MGI:J:83449
    Blood 101(10):3901-7

    2003
    Supplementation of diets with the black rice pigment fraction attenuates atherosclerotic plaque formation in apolipoprotein e deficient mice.
    Xia M , et al.
    PubMed:12612147
    MGI:J:82218
    J Nutr 133(3):744-51

    2002
    Hypobetalipoproteinemic Mice with a Targeted Apolipoprotein (Apo) B-27.6-specifying Mutation. IN VIVO EVIDENCE FOR AN IMPORTANT ROLE OF AMINO ACIDS 1254-1744 OF ApoB IN LIPID TRANSPORT AND METABOLISM OF THE ApoB-CONTAINING LIPOPROTEIN.
    Chen Z , et al.
    PubMed:11839763
    MGI:J:76047
    J Biol Chem 277(16):14135-45

    2002
    Dietary restriction reduces atherosclerosis and oxidative stress in the aorta of apolipoprotein E-deficient mice.
    Guo Z , et al.
    PubMed:12044962
    MGI:J:76930
    Mech Ageing Dev 123(8):1121-31

    2002
    Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI.
    Holm TM , et al.
    PubMed:11861300
    MGI:J:75090
    Blood 99(5):1817-24

    2002
    The ATP binding cassette transporter A1 (ABCA1) modulates the development of aortic atherosclerosis in C57BL/6 and apoE-knockout mice.
    Joyce CW , et al.
    PubMed:11752403
    MGI:J:73704
    Proc Natl Acad Sci U S A 99(1):407-12

    2002
    LDL receptor but not apolipoprotein E deficiency increases diet-induced obesity and diabetes in mice.
    Schreyer SA , et al.
    PubMed:11739102
    MGI:J:75609
    Am J Physiol Endocrinol Metab 282(1):E207-14

    2002
    Loss of Lymphotoxin-alpha but Not Tumor Necrosis Factor-alpha Reduces Atherosclerosis in Mice.
    Schreyer SA , et al.
    PubMed:11809756
    MGI:J:75788
    J Biol Chem 277(14):12364-8

    2002
    Reduction of blood pressure, plasma cholesterol, and atherosclerosis by elevated endothelial nitric oxide.
    van Haperen R , et al.
    PubMed:12364322
    MGI:J:80701
    J Biol Chem 277(50):48803-7

    2001
    Atherosclerosis in apoE knockout mice infected with multiple pathogens.
    Burnett MS , et al.
    PubMed:11120928
    MGI:J:66879
    J Infect Dis 183(2):226-231

    2001
    The adhesion receptor CD44 promotes atherosclerosis by mediating inflammatory cell recruitment and vascular cell activation.
    Cuff CA , et al.
    PubMed:11581304
    MGI:J:72315
    J Clin Invest 108(7):1031-40

    2001
    Dietary gamma-linolenic acid suppresses aortic smooth muscle cell proliferation and modifies atherosclerotic lesions in apolipoprotein E knockout mice.
    Fan YY , et al.
    PubMed:11385052
    MGI:J:69821
    J Nutr 131(6):1675-81

    2001
    Apoprotein C-III deficiency markedly stimulates triglyceride secretion in vivo: comparison with apoprotein E.
    Hirano T , et al.
    PubMed:11551841
    MGI:J:72104
    Am J Physiol Endocrinol Metab 281(4):E665-9

    2001
    Retrovirus-mediated expression of apolipoprotein A-I in the macrophage protects against atherosclerosis in vivo.
    Ishiguro H , et al.
    PubMed:11477092
    MGI:J:71811
    J Biol Chem 276(39):36742-8

    2001
    Mixed allogeneic chimerism with wild-type strains ameliorates atherosclerosis in apolipoprotein E-deficient mice.
    Ishimori N , et al.
    PubMed:11358981
    MGI:J:69647
    J Leukoc Biol 69(5):732-40

    2001
    Iron overload diminishes atherosclerosis in apoE-deficient mice.
    Kirk EA , et al.
    PubMed:11413162
    MGI:J:70613
    J Clin Invest 107(12):1545-53

    2001
    Compared with saturated fatty acids, dietary monounsaturated fatty acids and carbohydrates increase atherosclerosis and VLDL cholesterol levels in LDL receptor-deficient, but not apolipoprotein E-deficient, mice.
    Merkel M , et al.
    PubMed:11606787
    MGI:J:72580
    Proc Natl Acad Sci U S A 98(23):13294-9

    2001
    Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice.
    Miura Y , et al.
    PubMed:11208934
    MGI:J:66898
    J Nutr 131(1):27-32

    2001
    Pathophysiology of apolipoprotein E deficiency in mice: relevance to apo E-related disorders in humans.
    Moghadasian MH , et al.
    PubMed:11726538
    MGI:J:73202
    FASEB J 15(14):2623-30

    2001
    ApoE(-/-) mice develop atherosclerosis in the absence of complement component C5.
    Patel S , et al.
    PubMed:11485323
    MGI:J:71193
    Biochem Biophys Res Commun 286(1):164-70

    2001
    Impact of apoE deficiency on oxidative insults and antioxidant levels in the brain.
    Ramassamy C , et al.
    PubMed:11165374
    MGI:J:67204
    Brain Res Mol Brain Res 86(1-2):76-83

    2001
    Effect of azithromycin on murine arteriosclerosis exacerbated by Chlamydia pneumoniae.
    Rothstein NM , et al.
    PubMed:11120929
    MGI:J:66880
    J Infect Dis 183(2):232-238

    2000
    Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice [see comments]
    Febbraio M , et al.
    PubMed:10772649
    MGI:J:61672
    J Clin Invest 105(8):1049-56

    2000
    Age-dependent cytokine responses: trimethyltin hippocampal injury in wild-type, APOE knockout, and APOE4 mice.
    Harry GJ , et al.
    PubMed:11120597
    MGI:J:66874
    Brain Behav Immun 14(4):288-304

    2000
    Role of endogenous nitric oxide in progression of atherosclerosis in apolipoprotein E-deficient mice.
    Kauser K , et al.
    PubMed:10775149
    MGI:J:62352
    Am J Physiol Heart Circ Physiol 278(5):H1679-85

    2000
    Combined serum paraoxonase knockout/apolipoprotein E knockout mice exhibit increased lipoprotein oxidation and atherosclerosis.
    Shih DM , et al.
    PubMed:10748217
    MGI:J:62750
    J Biol Chem 275(23):17527-35

    1998
    The brain renin-angiotensin system contributes to the hypertension in mice containing both the human renin and human angiotensinogen transgenes.
    Davisson RL , et al.
    PubMed:9815152
    MGI:J:79945
    Circ Res 83(10):1047-58

    1998
    Apolipoprotein-E deficiency results in an altered stress responsiveness in addition to an impaired spatial memory in young mice.
    Zhou Y , et al.
    PubMed:9554991
    MGI:J:47604
    Brain Res 788(1-2):151-9

    1994
    The two-receptor model of lipoprotein clearance: tests of the hypothesis in knockout mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins.
    Ishibashi S , et al.
    PubMed:8183926
    MGI:J:18138
    Proc Natl Acad Sci U S A 91(10):4431-5

    1992
    Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E.
    Zhang SH , et al.
    PubMed:1411543
    MGI:J:16573
    Science 258(5081):468-71

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    等位基因参考文献


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