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C57BL/6J-ApcMin/J
品系货号:002020 | 通用名称:多发性肠道肿瘤小鼠
又名:Min小鼠
此品系杂合小鼠会出现贫血,并对自发性肠道腺瘤高度易感。
C57BL/6J-ApcMin/J 纯合小鼠不能存活。 此品系小鼠结直肠腺瘤发病率较高,因而适合用作结肠癌模型。少数 C57BL/6J-ApcMin 杂合雌鼠会出现乳腺肿瘤。
品系特点
化学诱导突变
  
同源
  
品系捐赠者 Alexandra Shedlovsky博士-威斯康星大学麦迪逊分校William Dove博士-威斯康星大学麦迪逊分校
  
原始参考文献
Info 如使用多发性肠道肿瘤小鼠模型(Min)开展研究,请在发表文章时引用原始文献,并在材料与方法部分注明JAX品系号002020。
查阅参考文献 >
  
品系详情
C57BL/6J-ApcMin/J 品系小鼠对自发性肠道腺瘤高度易感。纯合小鼠不能存活。根据最初的研究报告,饲喂高脂饲料的 C57BL/6J-ApcMin所有(100%)杂合小鼠在整个肠道中均出现超过 30 个腺瘤,大多数小鼠在 120 日龄前死亡。杂合小鼠还会出现贫血。(Moser et al., 1990, Su et al., 1992)。少数 C57BL/6J-ApcMin杂合雌鼠会出现乳腺肿瘤。后续报道表明,此品系可能携带显性突变 (Mom2) 基因,可减少 C57BL/6J-ApcMin杂合小鼠的息肉形成数量和发生率 (Silverman et al., 2002)。
此品系小鼠在运输时带有RapID耳标。 点击了解有关RapID耳标的更多信息。
品系建立
对 C57BL/6J 首建鼠使用 N-乙基-N-亚硝基脲 (N-ethyl-N-nitrosourea, ENU) 处理以诱导突变后,经正向遗传筛选,鉴定出一只 AKR/JxB6 F1 雌鼠有转圈行为。使这只雌鼠与 C57BL/6J 雄鼠交配。此回交产生的后代小鼠中,有些出现了成年发病的贫血和多发性肠道肿瘤 (multiple intestinal neoplasia, Min)。研究发现,抑癌基因 Apc(adenomatosis polyposis coli, 结肠腺瘤样息肉蛋白)与Min表型共分离。Apc的突变在结直肠癌和家族性腺瘤性息肉病 (familial adenomatous polyposis, FAP) 中被鉴定发现。Apc测序鉴定出第2549位核苷酸从T变为A,该突变使得亮氨酸变为终止密码子。经确定,转圈行为是独立的遗传性状,可通过与C57BL/6J后续杂交来消除。这些 ApcMin 小鼠在 1992 年被送往杰克森实验室时,维持着 C57BL/6J 基因背景。
参考文献
精选参考文献
如使用多发性肠道肿瘤小鼠模型(Min)开展研究,请在发表文章时引用原始文献,并在材料与方法部分注明JAX品系号002020。
1992

Multiple intestinal neoplasia caused by a mutation in the murine homolog of the APC gene [published erratum appears in Science 1992 May 22;256(5060):1114]

Su LK , et al.

PubMed:1350108

MGI:J:830

Science 256(5057):668-70

1990

A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.

Moser AR , et al.

PubMed:2296722

MGI:J:10209

Science 247(4940):322-4

基因分型和繁育
Wild-type from the colony
Allele Symbol Apc<min>
Allele Specific Protocols
  
此品系通过将杂合雄鼠与 C57BL/6J 雌鼠交配来维持。不建议使用杂合雌鼠,因为贫血和肠道腺瘤会干扰妊娠。出现贫血和肿瘤后,杂合雄鼠的繁育性能也会随之下降。
繁育策略
近交系 X 杂合子: C57BL/6J x 杂合子
销售和使用条款

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002020
多发性肠道肿瘤小鼠
肿瘤发生,转移,以及肿瘤免疫等研究领域,尤其是肠道肿瘤
参考文献|
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  •  
    精选参考文献
    如使用多发性肠道肿瘤小鼠模型(Min)开展研究,请在发表文章时引用原始文献,并在材料与方法部分注明JAX品系号002020。

    1990
    A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.
    Moser AR , et al.
    PubMed:2296722
    MGI:J:10209
    Science 256(5057):668-70

    1992
    Multiple intestinal neoplasia caused by a mutation in the murine homolog of the APC gene [published erratum appears in Science 1992 May 22;256(5060):1114]
    Su LK , et al.
    PubMed:1350108
    MGI:J:830
    Science 247(4940):322-4

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    其它参考文献

    2004
    Apc10.1: an ApcMin/+ intestinal cell line with retention of heterozygosity.
    De Giovanni C , et al.
    PubMed:14750170
    MGI:J:88314
    Int J Cancer 109(2):200-6

    2004
    Activation of nuclear hormone receptor peroxisome proliferator-activated receptor-delta accelerates intestinal adenoma growth.
    Gupta RA , et al.
    PubMed:14758356
    MGI:J:88127
    Nat Med 10(3):245-7

    2004
    Prevention of gastrointestinal tumors based on adenomatous polyposis coli gene mutation by dendritic cell vaccine.
    Iinuma T , et al.
    PubMed:15124022
    MGI:J:90168
    J Clin Invest 113(9):1307-17

    2004
    Suppression of intestinal polyposis in Apcmin/+ mice by targeting the nitric oxide or poly(ADP-ribose) pathways.
    Mabley JG , et al.
    PubMed:15063141
    MGI:J:89155
    Mutat Res 548(1-2):107-16

    2004
    Adenomatous polyposis coli truncation mutations in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-induced intestinal tumours of multiple intestinal neoplasia mice.
    Mollersen L , et al.
    PubMed:14706516
    MGI:J:87470
    Mutat Res 557(1):29-40

    2004
    The anti-ischemia agent ranolazine promotes the development of intestinal tumors in APC(Min/+) mice.
    Suckow MA , et al.
    PubMed:15159018
    MGI:J:90679
    Cancer Lett 209(2):165-9

    2004
    Dietary Soy Sphingolipids Suppress Tumorigenesis and Gene Expression in 1,2-Dimethylhydrazine-Treated CF1 Mice and Apc(Min/+) Mice.
    Symolon H , et al.
    PubMed:15113963
    MGI:J:90172
    J Nutr 134(5):1157-1161

    2004
    Prostaglandin E(2) promotes colorectal adenoma growth via transactivation of the nuclear peroxisome proliferator-activated receptor delta.
    Wang D , et al.
    PubMed:15380519
    MGI:J:93551
    Cancer Cell 6(3):285-95

    2004
    Dietary resveratrol does not affect intestinal tumorigenesis in Apc(Min/+) mice.
    Ziegler CC , et al.
    PubMed:14704285
    MGI:J:88124
    J Nutr 134(1):5-10

    2003
    Effects of a glycogen synthase kinase-3 inhibitor, lithium, in adenomatous polyposis coli mutant mice.
    Gould TD , et al.
    PubMed:12770514
    MGI:J:84200
    Pharmacol Res 48(1):49-53

    2003
    Thrombospondin 1--a regulator of adenoma growth and carcinoma progression in the APC(Min/+) mouse model.
    Gutierrez LS , et al.
    PubMed:12584168
    MGI:J:79641
    Carcinogenesis 24(2):199-207

    2003
    Reduction in alkaline sphingomyelinase in colorectal tumorigenesis is not related to the APC gene mutation.
    Hertervig E , et al.
    PubMed:12774245
    MGI:J:84197
    Int J Colorectal Dis 18(4):309-13

    2003
    Calorie restriction and diet composition modulate spontaneous intestinal tumorigenesis in Apc(Min) mice through different mechanisms.
    Mai V , et al.
    PubMed:12702556
    MGI:J:82980
    Cancer Res 63(8):1752-5

    2003
    Concomitant suppression of hyperlipidemia and intestinal polyp formation in Apc-deficient mice by peroxisome proliferator-activated receptor ligands.
    Niho N , et al.
    PubMed:14522940
    MGI:J:86036
    Cancer Res 63(18):6090-5

    2003
    Impact of Dnmt1 deficiency, with and without low folate diets, on tumor numbers and DNA methylation in Min mice.
    Oikarinen S , et al.
    PubMed:12844383
    MGI:J:85024
    Br J Nutr 90(1):119-25

    2003
    Transcriptional profiling of the transition from normal intestinal epithelia to adenomas and carcinomas in the APCMin/+ mouse.
    Paoni NF , et al.
    PubMed:13130079
    MGI:J:86113
    Physiol Genomics 15(3):228-35

    2003
    Exclusion of Madh2, Madh4, and Madh7 as candidates for the modifier of Min 2 ( Mom2) locus.
    Silverman KA , et al.
    PubMed:12584607
    MGI:J:81868
    Mamm Genome 14(2):119-29

    2003
    Msh2 deficiency enhances somatic Apc and p53 mutations in Apc+/-Msh2-/- mice.
    Sohn KJ , et al.
    PubMed:12584170
    MGI:J:79407
    Carcinogenesis 24(2):217-24

    2003
    Thrombospondin 1--a regulator of adenoma growth and carcinoma progression in the APC(Min/+) mouse model.
    Trasler J , et al.
    PubMed:12538347
    MGI:J:81577
    Carcinogenesis 24(1):39-45

    2003
    Cooperative effects of matrix metalloproteinase and cyclooxygenase-2 inhibition on intestinal adenoma reduction.
    Wagenaar-Miller RA , et al.
    PubMed:12778076
    MGI:J:83488
    Br J Cancer 88(9):1445-52

    2003
    Suppression of intestinal polyposis in Mdr1-deficient ApcMin/+ mice.
    Yamada T , et al.
    PubMed:12615699
    MGI:J:82288
    Cancer Res 63(5):895-901

    2003
    Regional response leading to tumorigenesis after sulindac in small and large intestine of mice with Apc mutations.
    Yang K , et al.
    PubMed:12663524
    MGI:J:82832
    Carcinogenesis 24(3):605-11

    2002
    One dose of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) or 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) induces tumours in Min/+ mice by truncation mutations or LOH in the Apc gene.
    Andreassen A , et al.
    PubMed:12034317
    MGI:J:77184
    Mutat Res 517(1-2):157-66

    2002
    Selenium-enriched broccoli decreases intestinal tumorigenesis in multiple intestinal neoplasia mice.
    Davis CD , et al.
    PubMed:11823596
    MGI:J:74364
    J Nutr 132(2):307-9

    2002
    Selenium-enriched broccoli decreases intestinal tumorigenesis in multiple intestinal neoplasia mice.
    Davis CD , et al.
    PubMed:11823596
    MGI:J:74364
    J Nutr 132(2):307-9

    2002
    Absence of Post-translational Aspartyl beta -Hydroxylation of Epidermal Growth Factor Domains in Mice Leads to Developmental Defects and an Increased Incidence of Intestinal Neoplasia.
    Dinchuk JE , et al.
    PubMed:11773073
    MGI:J:75888
    J Biol Chem 277(15):12970-7

    2002
    Genetic and molecular control of folate-homocysteine metabolism in mutant mice.
    Ernest S , et al.
    PubMed:12016514
    MGI:J:76559
    Mamm Genome 13(5):259-67

    2002
    Vaccine-based therapy directed against carcinoembryonic antigen demonstrates antitumor activity on spontaneous intestinal tumors in the absence of autoimmunity.
    Greiner JW , et al.
    PubMed:12460911
    MGI:J:80324
    Cancer Res 62(23):6944-51

    2002
    Prostaglandin E(2) protects intestinal tumors from nonsteroidal anti-inflammatory drug-induced regression in Apc(Min/+) mice.
    Hansen-Petrik MB , et al.
    PubMed:11809688
    MGI:J:74005
    Cancer Res 62(2):403-8

    2002
    Colonoscopy in mice.
    Huang EH , et al.
    PubMed:11961598
    MGI:J:77864
    Surg Endosc 16(1):22-4

    2002
    1alpha,25-(OH)(2)-D(3) and its synthetic analogue decrease tumor load in the Apc(min) Mouse.
    Huerta S , et al.
    PubMed:11830528
    MGI:J:74361
    Cancer Res 62(3):741-6

    2002
    Arachidonic and linoleic acid metabolism in mouse intestinal tissue: evidence for novel lipoxygenase activity.
    Kawajiri H , et al.
    PubMed:11811948
    MGI:J:74371
    Arch Biochem Biophys 398(1):51-60

    2002
    Reduction of Intestinal Neoplasia With Adenomatous Polyposis Coli Gene Replacement and COX-2 Inhibition Is Additive.
    Lew JI , et al.
    PubMed:12127122
    MGI:J:77954
    J Gastrointest Surg 6(4):563-8

    2002
    Reduction of Intestinal Neoplasia With Adenomatous Polyposis Coli Gene Replacement and COX-2 Inhibition Is Additive.
    Lew JI , et al.
    PubMed:12127122
    MGI:J:77954
    J Gastrointest Surg 6(4):563-8

    2002
    Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors.
    Lustig B , et al.
    PubMed:11809809
    MGI:J:74286
    Mol Cell Biol 22(4):1184-93

    2002
    Detection of dysplastic intestinal adenomas using enzyme-sensing molecular beacons in mice.
    Marten K , et al.
    PubMed:11832455
    MGI:J:74358
    Gastroenterology 122(2):406-14

    2002
    Chemopreventive efficacy and pharmacokinetics of curcumin in the min/+ mouse, a model of familial adenomatous polyposis.
    Perkins S , et al.
    PubMed:12050094
    MGI:J:77168
    Cancer Epidemiol Biomarkers Prev 11(6):535-40

    2002
    Therapeutic utility of aspirin in the ApcMin/+ murine model of colon carcinogenesis.
    Reuter BK , et al.
    PubMed:12171603
    MGI:J:78510
    BMC Cancer 2(1):19

    2002
    Studies of methionine cycle intermediates (SAM, SAH), DNA methylation and the impact of folate deficiency on tumor numbers in Min mice.
    Sibani S , et al.
    PubMed:11756224
    MGI:J:73559
    Carcinogenesis 23(1):61-5

    2002
    Enhanced colon carcinogenesis induced by azoxymethane in min mice occurs via a mechanism independent of beta-catenin mutation.
    Suzui M , et al.
    PubMed:12049812
    MGI:J:77169
    Cancer Lett 183(1):31-41

    2002
    Response to 5-fluorouracil chemotherapy is modified by dietary folic acid deficiency in Apc(Min/+) mice.
    Tucker J , et al.
    PubMed:12359363
    MGI:J:79692
    Cancer Lett 187(1-2):153

    2002
    Absence of the CD44 gene prevents sarcoma metastasis.
    Weber GF , et al.
    PubMed:11956084
    MGI:J:76201
    Cancer Res 62(8):2281-6

    2002
    Microadenomatous lesions involving loss of apc heterozygosity in the colon of adult apc(min/+) mice.
    Yamada Y , et al.
    PubMed:12438216
    MGI:J:80301
    Cancer Res 62(22):6367-70

    2001
    Intestinal tumours induced by the food carcinogen 2-amino-1-methyl-6-phenylimidazo.
    Andreassen A , et al.
    PubMed:11420398
    MGI:J:70642
    Mutagenesis 16(4):309-15

    2001
    Haptoglobin, inflammation, and tumorigenesis in the MIN mouse.
    Barbour K W , et al.
    PubMed:11865977
    MGI:J:75249
    Redox Rep 6(6):366-8

    2001
    Mammary tumor induction and premature ovarian failure in ApcMin mice are not enhanced by Brca2 deficiency.
    Bennett LM , et al.
    PubMed:11215675
    MGI:J:67445
    Toxicol Pathol 29(1):117-25

    2001
    Qualitative and Quantitative Relationship between Dysplastic Aberrant Crypt Foci and Tumorigenesis in the Min/+ Mouse Colon.
    Erik Paulsen J , et al.
    PubMed:11431334
    MGI:J:70170
    Cancer Res 61(13):5010-5

    2001
    Deletion of cytosolic phospholipase A(2) suppresses Apc(Min)-induced tumorigenesis.
    Hong KH , et al.
    PubMed:11274413
    MGI:J:68495
    Proc Natl Acad Sci U S A 98(7):3935-9

    2001
    Suppression of intestinal polyps in msh2-deficient and non-msh2-deficient multiple intestinal neoplasia mice by a specific cyclooxygenase-2 inhibitor and by a dual cyclooxygenase-1/2 inhibitor.
    Lal G , et al.
    PubMed:11507063
    MGI:J:70902
    Cancer Res 61(16):6131-6

    2001
    Growth stimulation of intestinal tumours in Apc(Min/+) mice by dietary L-methionine supplementation.
    Paulsen JE , et al.
    PubMed:11848484
    MGI:J:75262
    Anticancer Res 21(5):3281-4

    2001
    Chemoprevention of intestinal tumorigenesis by nabumetone: induction of apoptosis and Bcl-2 downregulation.
    Roy HK , et al.
    PubMed:11355956
    MGI:J:69899
    Br J Cancer 84(10):1412-6

    2001
    Modulation of intracellular beta-catenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids.
    Schmelz EM , et al.
    PubMed:11559543
    MGI:J:71590
    Cancer Res 61(18):6723-9

    2001
    Intestinal tumorigenesis in multiple intestinal neoplasia mice induced by the food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine: perinatal susceptibility, regional variation, and correlation with DNA adducts.
    Steffensen IL , et al.
    PubMed:11751386
    MGI:J:73361
    Cancer Res 61(24):8689-96

    2001
    Combination cancer chemoprevention with green tea extract and sulindac shown in intestinal tumor formation in Min mice.
    Suganuma M , et al.
    PubMed:11206275
    MGI:J:67939
    J Cancer Res Clin Oncol 127(1):69-72

    2001
    (+)-Catechin inhibits intestinal tumor formation and suppresses focal adhesion kinase activation in the min/+ mouse.
    Weyant MJ , et al.
    PubMed:11196148
    MGI:J:67060
    Cancer Res 61(1):118-25

    2001
    Reciprocal expression of ERalpha and ERbeta is associated with estrogen-mediated modulation of intestinal tumorigenesis.
    Weyant MJ , et al.
    PubMed:11289129
    MGI:J:68469
    Cancer Res 61(6):2547-51

    2001
    Differential dietary effects on colonic and small bowel neoplasia in C57BL/6J Apc Min/+ mice.
    Yu CF , et al.
    PubMed:11478486
    MGI:J:70920
    Dig Dis Sci 46(7):1367-80

    2000
    Beef induces and rye bran prevents the formation of intestinal polyps in Apc(Min) mice: relation to beta-catenin and PKC isozymes.
    Mutanen M , et al.
    PubMed:10837006
    MGI:J:62978
    Carcinogenesis 21(6):1167-73

    2000
    Chemopreventive activity of hydroksymatairesinol in adenomatous polyposis colimultiple intestinal neoplasia (Apc)(Min) mice
    Oikannen SI , et al.
    PubMed:10996730
    MGI:J:64666
    Cancer Lett 159(2):183-7

    2000
    Identification and quantification of aberrant crypt foci in the colon of Min mice--a murine model of familial adenomatous polyposis
    Paulsen JE , et al.
    PubMed:10868458
    MGI:J:62966
    Scand J Gastroenterol 35(5):534-9

    2000
    Chemoprevention of familial adenomatous polyposis development in the APC(min) mouse model by 1,4-phenylene bis(methylene)selenocyanate.
    Rao CV , et al.
    PubMed:10753194
    MGI:J:61783
    Carcinogenesis 21(4):617-21

    2000
    Uroguanylin treatment suppresses polyp formation in the Apc(Min/+) mouse and induces apoptosis in human colon adenocarcinoma cells via cyclic GMP.
    Shailubhai K , et al.
    PubMed:11016642
    MGI:J:64784
    Cancer Res 60(18):5151-7

    2000
    Effects of dietary folate on intestinal tumorigenesis in the apcMin mouse
    Song J , et al.
    PubMed:11034085
    MGI:J:65027
    Cancer Res 60(19):5434-40

    1997
    Interleukin 12 (IL-12) is crucial to the development of protective immunity in mice intravenously infected with mycobacterium tuberculosis.
    Cooper AM , et al.
    PubMed:9206995
    MGI:J:41494
    J Exp Med 186(1):39-45

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    等位基因参考文献


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外观
详情

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Related Genotype: a/a